Journal: Microbiology Spectrum

Article Title: A novel role of Dermatophagoides farinae -derived miR-276-3p in aggravating mite-induced allergic airway inflammation

doi: 10.1128/spectrum.01923-25

Figure Lengend Snippet: Dfa-miR-276-3p as a prime exacerbates DFE-induced airway inflammation in vivo . ( A ) Schematic diagram for construction of AAV-dfa-miR-276-3p-overexpressing mouse models. ( B ) Immunofluorescence assay detects co-expression of EGFP (green) and Claudin-1 (red) in mouse lung tissues, and the nuclei are stained with DAPI (blue) ( n = 3). Scale bar = 50 μm. ( C ) qPCR assay quantifies EGFP mRNA expression in mouse lung tissues. ( D ) qPCR assay quantifies dfa-miR-276-3p expression in mouse lung tissues. ( E ) Schematic diagram for construction of a mouse model of airway sensitization following AAV-dfa-miR-276-3p overexpression in lungs. ( F ) Total cell counts in mouse BALF ( n = 3). ( G ) ELISA measures IL-4 levels in mouse BALF ( n = 3). ( H ) ELISA measures IL-5 levels in mouse BALF ( n = 3). ( I ) ELISA measures IgE antibody levels in mouse serum samples ( n = 6). ( J ) ELISA measures IL-10 levels in mouse BALF ( n = 3). ( K ) HE and PAS staining of representative lung sections ( n = 3). Scale bar = 100 μm. ns, not significant, * P < 0.05, ** P < 0.01, and *** P < 0.001. Data are presented as means ± SEM. Difference of means among groups is tested for statistical significance with ANOVA, followed by Tukey’s post hoc test.

Article Snippet: Sections or cell culture slides were blocked with 10% goat serum at room temperature, and the cell culture slides were incubated with the primary anti-NF-κB p65 antibody (Cell Signaling Technology, Inc.), while the lung tissue sections were incubated with the primary anti-claudin 1 antibody (Proteintech, Manchester, UK).

Techniques: In Vivo, Immunofluorescence, Expressing, Staining, Over Expression, Enzyme-linked Immunosorbent Assay

Journal: Microbiology Spectrum

Article Title: A novel role of Dermatophagoides farinae -derived miR-276-3p in aggravating mite-induced allergic airway inflammation

doi: 10.1128/spectrum.01923-25

Figure Lengend Snippet: Dfa-miR-276-3p promotes DFE-induced airway inflammation through inhibiting STC1 expression to regulate ROS/NF-κB pathway in vivo . ( A ) Schematic diagram for different treatments of mice. ( B ) Total cell counts in mouse BALF ( n = 3). ( C ) ELISA measures IL-4 levels in mouse BALF ( n = 3). ( D ) ELISA measures IL-5 levels in mouse BALF ( n = 3). ( E ) ELISA measures IgE antibody levels in mouse serum samples ( n = 6). ( F ) ELISA measures IL-10 levels in mouse BALF ( n = 3). ( G ) HE and PAS staining of representative lung sections ( n = 3). Scale bar = 100 μm. ( H ) ROS levels in mouse lung tissues ( n = 6). ( I and J ) Western blotting determines STC1, NF-κB p65, and p-p65 protein expression in mouse lung tissues ( n = 3). ns, not significant, * P < 0.05, ** P < 0.01, and *** P < 0.001. Data are presented as means ± SEM. Difference of means among groups was tested for statistical significance with ANOVA, followed by Tukey’s post hoc test.

Article Snippet: Sections or cell culture slides were blocked with 10% goat serum at room temperature, and the cell culture slides were incubated with the primary anti-NF-κB p65 antibody (Cell Signaling Technology, Inc.), while the lung tissue sections were incubated with the primary anti-claudin 1 antibody (Proteintech, Manchester, UK).

Techniques: Expressing, In Vivo, Enzyme-linked Immunosorbent Assay, Staining, Western Blot